Research article

STUDY OF THE EFFECT OF JASMONIC ACID (JA) AND NANO-FERTILIZER ON QUALITATIVE INDICATORS AND SOME ACTIVE COMPOUNDS IN THE LEAVES OF THREE CULTIVARS OF THE ROCKET PLANT ERUCA SATIVA MILL.

Hisham Aziz Amran* and Jamal Ahmed Abbass

Online First: December 21, 2022


An experiment was carried out in the Orchards and Nurseries Development Project - Karbala Agriculture Directorate for the two agricultural seasons 2020-2021 and 2021-2022 to study the physiological effects of jasmonic acid (JA) and nano-fertilizer on qualitative indicators and some active substances for the leaves of three cultivars of Rocket plant. The experiment was designed according to the Split Split Plot Design System and using the Randomized Complete Block Design (R.C.B.D), and the experiment included 27 treatments distributed randomly and with three replicates (81 experimental units) from three factors, the main factor is three varieties of Rocket plant, one of them French variety and the other Turkish variety and comparing them with the local variety, and the secondary factor is NPK nano fertilizer (0, 2, 4) gm. L-1, while the sub-secondary factor is the growth regulator jasmonic acid (0, 15, 30) mg. L-1, ANOVA was performed, then the rates were compared according to the comparison between the means using Duncan's Multiple Range Test at a probability level of 0.05, and the data were analyzed by the statistical program GenStat Release 12.1, the most important results are summarized as follows: Spraying with acid Jasmonic and nano-fertilizer on the leaves of the three cultivars of Rocket plant showed significant differences in the studied indicators, as the local cultivar that sprayed its plants with nano-fertilizer significantly outperformed 4 g. L-1 and jasmonic acid 30 mg.L-1 V1N2J2, which gave the highest results for both seasons of the experiment compared to plants of the other two cultivars in all qualitative indicators of the plant, as it reached in the leaf content indicators of total chlorophyll (123.88 and 113.68 mg.100). gm-1 fresh weight), the percentage of total soluble carbohydrates (9.25 and 9.17%) and ascorbic acid (128.76 and 126.72 mg.L-1 and jasmonic acid 30 mg.L-1 V2N2J2 significantly outperformed the two indexes of the leaves content of the active compounds, as it reached in the leaves content of glucosinolate (9.98 and 11.89 mg.g-1 dry weight) and glutathione (4.05 and 3.99 mg.g- 1 dry weight) sequentially for both seasons of the experiment.

Keywords

Rocket varieties, Nano fertilizer, jasmonic acid, qualitative indicators and active ingredients in leaves.