Research article


Arnos E. T1, AL-Miahy Falah H.1 and AL-Hussaini Z.A,2

Online First: December 21, 2022

Random Amplified Polymorphic DNA (RAPD) technique was applied to study the genetic stability using 5 random primers (A15, D2, F8, F18 and H9) in microtuber production stage of three cultivars of potato (Arnova, Rivera and Provento) in vitro propagated for 3 sub culture) ( sub culture was repeated every 4 weeks) and interaction with medium (0.0 and 1.0 mg L-1 IAA) and growth regulators (4 mg L-1 BA, 4 mg L-1 Kin-1 and 4 mg L- 1 BA + 4 mg L-1 Kin). Cultures were incubated at a temperature of 25 ± 2 °C and 16 hours of illumination day-1 for 10 days, then the cultures were incubated at a temperature of 17 ± 2 °C in the dark for three months. Data were taken on the number of microtuber, their diameters and weights. The results showed the superiority of Riviera plants grown in the medium supplemented with 1 mg L-1 IAA + 4 mg L-1 Kin in the number of microtubers at rate 2.0 tuber plant-1, and the Provento cultivar plants grown in the medium with 1 mg L-1 IAA. + 4 mg L-1 Kin in the diameter of the microtubers and their weights at rates 0.666 cm and 0.440 g, respectively. All primers (A15, D2, F8, F12 and H9) produced a number of monomorphic bands 21, 19 and 17 bands for all plants (in vitro propagated and mother plants) of the cultivars of potato Arnova, Riviera and Provento, respectively, where the percentage of bands similarity was reached 100 %, which indicated its genetic stability and no difference from the mother plants and thus the efficiency of obtaining plants identical to the original plant by using the plant tissue culture technique.


Potato, microtubers, genetic stability and RAPD