STUDYING THE EFFICIENCY OF BIOREMEDIATION IN REMOVING LEAD FROM CONTAMINATED SOILS USING THE PSEUDOMONAS OLEOVORANS BACTERIUM

The current study included a laboratory biological experiment, which was conducted in the laboratories of the Department of Life Sciences–College of Education for Pure Sciences / University of Anbar from November to January 2021.The purpose of this experiment was isolating and purifying bacteria and improving the environmental conditions for the growth and tolerance of bacteria to the element of lead added to the culture medium. A total of (10) bacterial isolates were obtained and isolated from soil contaminated with heavy elements. These were subjected to primary and secondary screening and one of them was selected as efficient and potential isolation for concentration of 750 mg/L of lead element and was diagnosed using the Vitek compact2 system. Results for improved environmental conditions for the growth of lead-induced elected bacterial isolation Pseudomonas oleovorans (250,500,750, 1,000 mg/L) showed that the best bacterial growth density was at pH 6 and using 3 ml/100 ml of the agricultural medium which had a vaccine size (25 × 105 cells/mL of bacterial cells). Best incubation time was obtained at 96 hours and a temperature of 30 °C was the best conditions for elected bacterial isolation, while the highest concentration was at 750 mg/l.