A NOVEL ELECTROCHEMICAL ASSAY BASED BIOLOGICAL SYNTHESIS NANOPARTICLES FOR SOME HYDROCARBONS USING SINGLE-STRANDED DNA-BINDING APTAMER

Water samples were collected from Hammar Mushrif plant from three stages to determine the hydrocarbon compounds .A novel electrochemical treatment method was fabricated and modified for rapid, highly sensitive and specific detection of hydrocarbons based on aptamer probe as single-stranded DNA-binding nanoreceptors with electroactive redox probe (Ferrocene) to the surface of screen-printed gold electrode. The results showed six PAHs compounds (Anthracene, B (K) Fluoranthene, IND (1, 2, 3-CD) +Di, B (B) Fluoranthene, pyrene, B (A) pyrene) which appeared in three stations before nanotreatment in station 1 their concentrations were (619.71, 23.375, 114.22, 41.221, 17.862, 48.422 mg/l), and in station 2 the concentrations were (488.26, 16.582, 105, 39.509, 18.575, 17.017), while in station 3 the concentrations were (295.31, 13.334, 88.43, 17.336, 10.864, 10.972). The treatment efficiency rang was (2.79% -100%).The designed electrochemical assay exhibited high specificity toward hydrocarbons. Moreover, the developed treating method was successfully applied for removing of target analytes which present in water samples polluted with hydrocarbons.