Research article


1 Athraa Abass Esmiel , 2Huda Nsaif Jasim

Online First: December 16, 2022

P. mirabilis have been linked to food poisoning outbreaks, it is a pathogenic bacteria that can contaminate chicken meat not just through handling, preparation, and processing, but also by washing carcasses with polluted water. This study was conducted in the Department of Veterinary Public Health / Laboratory of Meat Hygiene from September (2020) to January (2021), where samples of chicken meat were collected under chilled conditions from Baghdad's local markets, and all health aspects were considered when transferring these samples to the laboratory .this study includes the collecting of 200 samples as well as human urine and feces samples from various sites in Baghdad city. Proteus mirabilis was identified from 38 of 200 samples (19%) and isolates from human (urine and feces). We detected a strain of P. mirabilis isolated from chicken meat and patients' specimens (urine and feces) in Baghdad. To confirm the diagnosis API 20E kits, Vitek2 system, and ultimately the presumptive diagnosis was validated by utilizing PCR test. Proteus mirabilis isolates were diagnosed using PCR by 16S ribosomal RNA (16S rRNA) primers to determine their gastrointestinal pathogenicity and relationship with UTI infection experiments were performed to compare P. mirabilis strain (isolated from chicken meat carcasses) and P. mirabilis (isolated from human fecal and urine) by 16S ribosomal RNA gene, partial sequence to show genetic similarity between two strains, Also, we recorded Iraqi sequences in NCBI-Gene-Bank and DDBJ of INSDC (USA). Sequencing technology was used to diagnose Proteus mirabilis strain isolates were examined by (16SrRNA) genes, and the new isolates were recorded in Nucleotide/Blast and Iraqi were recorded as the first sequencing in Gene-Bank/NCBI, DDBJ, and ENA (INSDC), each sequence has Accession numbers as follows: (ID: OM811980.1, ID: OM811981.1). These are the first Iraqi isolates from chicken meat and human specimens to be recorded in the Gene Bank/USA, Also results shows genetic tree phylogenetic among P. mirabilis isolates in different countries in the world.


Proteus mirabilis, genetic similarity detection, (16SrRNA) genes,