HEMATOLOGICAL, BIOCHEMICAL AND DNA ANALYSIS OF GALANGIN IMMOBILIZED ON GOLD NANOPARTICLES

Galangin (GAL) is a natural flavonoid, has various pharmacological activities including antimicrobial, antioxidant, anti-inflammatory anti-cancer and anti-angiogenesis properties. In order to enhance GAL activities, in the current study, GAL was loaded on gold nanoparticles (Au-NPs-GAL) and free radical scavenging activity, DNA strand break damage and biocompatibility were evaluated. the results obtained from the DPPH assay showed that AuNPs-GAL has a significant efficiency than AuNPs and GAL in individual in inhibiting free radicals. Comet assays was carried out using two types of breast cancer cell line MCF-7and MDA-MB-231 cell lines, and the results indicated the highly significant damage caused by AuNPs-GAL compared to AuNPs and GAL, interestingly, the effects was higher on the MCF-7 than the MDA-MB-231 cell line. Hemolysis and aggregation of red blood cells (RBCs) results showed that AuNPs, AuNPs-GAL and free galangin have good biocompatibility and can be used safely inside the body according to ASTM standard. The current study’s findings indicated the safety of GAL along with its anti-cancer ability, however, additional research is needed to investigate the under lining therapeutic potential.