ANTIMICROBIAL AND CYTOTOXIC EFFECT OF CAMELLIA SINENSIS AND ACACIA NILOTICA HERBAL FORMULATION

INTRODUCTION:Camellia sinensis (Green tea) and their polyphenols, have attracted great attention over the years as possible nutraceuticals, because of their multiple bioactivities, especially antioxidant and anti-inflammatory activity, which could be exploited in several diseases, including such skin ailments. Acacia nilotica (Babool) has more medicinal uses and it has potent antioxidant activity. It provides nutrients and therapeutic ingredients for treatment of many diseases. The aim of the study is to evaluate the combined action of Camellia sinensis (Green tea) and Acacia nilotica (Babool) herbal formulation whether it has better antimicrobial and cytotoxic effect MATERIALS AND METHODS: 1g of green tea and babul powder was weighed aseptically and then dissolved in 100ml of distilled water. Then the solution is boiled for about 5 minutes at a temperature of about 60-80 degree celsius and then allowed to cool down followed by filtration of extract and then copper nanoparticles were synthesized and antimicrobial and cytotoxic activity was done. RESULTS: Antimicrobial activity was noted in different pathogens like E. faecalis, C.albicans, S.mutans and S. aureus. It was seen that there was an increased zone of inhibition seen in the control group of all pathogens. It has been observed that no change was observed in live nauplius in day 1 and after that differs in each concentration it was effective in 5μl than other concentrations. And there was no change observed in the control group. From the study it can be clearly seen that a concentration dependent Antimicrobial activity and cytotoxic effect seen which is evident in herbal formulation. CONCLUSION :It has been concluded that the green tea and babul herbal formulations have better antimicrobial and cytotoxic activity.