MOLECULAR IDENTIFICATION OF FIVE SPECIES OF FAMILY MUSCIDAE (INSECT:DIPTERA) FROM BASRAH, SOUTH IRAQ

Muscidae are a very large and diverse family, so it is difficult to identify them morphologically. Therefore, this study used PCR for five Muscidae species: Musca domestica, Musca sorbens, Muscina stabulans, Stomoxys calcitrans, and Lispe orientalis, The results of the study showed that the morphological definition matches the molecular definition whose genetic sequences matched globally equivalent species recorded in GenBank at rates ranging from (99.84% - 99.15%) . For the first time, the genetic sequence of each species was documented and preserved on the National Center for Informatics of Life Technology website NCBI under special accession numbers with information indicating the names of researchers and the place of collection. The branches of the phylogenetic tree showed the values of genetic distances for matching and affinity of species with their equivalent species from different geographical environments, and showed high genetic affinity with some species with a genetic distance equal to zero.