Research article

ANTIMICROBIAL ACTIVITY OF SOME LACTIC ACID BACTERIA ON THE SOME GRAM NEGATIVE BACTERIA

1 Yaqeen Dheyaa Faris and 2Ahmed Khassaf Atya

Online First: November 15, 2022


The current investigation was carried out in Nasiriya city hospitals over a four-month period, and samples of Gram-negative bacteria from skin burns and urinary tract infections as well as isolates of lactic acid bacteria from traditional cheese and cheese were collected. bird's waterfowl intestines This study's objective was to find out whether lactic acid bacteria could prevent Gram-negative pathogenic bacteria from growing. In the study, pathogenic bacteria were isolated and identified using standard culture media, microscopy, biochemical testing, and VITIC method, for the purpose of identifying lactic acid bacteria, MRS medium, microscopy, and biochemical assays were used, along with genetic sequencing, PCR identification, and microscopy. The isolates appear to be members of the Enterococcus spp (140) samples total, 90 from typical cheese, 20 of which were effective against harmful bacteria, and 50 from the intestines of ducks, with an effectiveness of 18 isolates, were used in the study. 70 samples of skin diseases were used to isolate pathological bacteria, 15 of which included gram-negative bacteria. Thirty-five samples of urinary tract infections were isolated, and 10 of those samples contained Gram-negative bacteria. E. coli had the highest rate of both diseases, with males ranking first for skin infections and females first for urinary tract infections. Regarding antibiotics, some of them were completely ineffective against germs, while others were just partially effective.

Keywords

Gram-negative bacteria, Lactic acid bacteria, Pseudomonas auroginosa, Escherichia coli, Proteus ,Serratia, Klebsiella