Research article

STUDY THE EFFECT OF ROSMARINUS OFFICINALIS L. ESSENTIAL OIL ON TYPE1&TYPE3 FIMBRIAE GENES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE

Zahraa Abdulrazak Jabar⃰ and Alyaa Razooqi Hussein

Online First: November 11, 2022


Klebsiella pneumoniae bacteria are opportunistic pathogen.This bacterium have the ability to produce biofilm which is a virulence factor .The aim of current project was to study the effect of rosemary essential oil on inhibition of biofilm in (Strong biofilm producer and multi drug resistance isolates) and also to evaluate the action of this oil in MICs concentrations in gene expression of (fimh and mrkd genes) for type 1 and type 3 fimbriae respectivelly against three isolates .the results of antibiofilm activity showed that there were a significant differences (P ≤ 0.05) in OD values of biofilm formation between K. pneumoniae isolates before and after treatment with Rosemary oil, and the biofilm inhibition % was also determined.The results of gene expression pre and post treatment with 1/5 MICs value of rosemary oil clarified that Rosmarinus officinalis L.essential oil showed different effects for gene expression for the three selected isolates(8,24and 40).

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