DETERMINATION OF THE INHIBITORY ACTIVITY OF GONDERMA EXTRACT AGAINST SOME TYPES OF BACTERIA THAT ARE RESISTANT TO MULTIPLE ANTIBIOTICS.

200 median urine samples were collected from patients and visitors to Kirkuk Hospital for the period between 11/18/2020 to 2/17/2021. The samples were taken from different age groups ranging from 20-50 years of both sexes. The results of bacterial culture showed that 138 samples and 69% of the samples gave bacteria growth on the media MacConkey agar, Mannitol salt agar, Blood agar, while 62 and 31% of the samples did not give bacteria growth. Using the Vitek 2 Compact System, 7 different types of Gram-negative bacteria and 9 different types of Gram-positive bacteria were diagnosed, which included 25 Escherichia isolates. coli by 18.8%, followed by Staphylococcus aureus and its isolates numbered 20 by 14.4%, and Staphylococcus bacteria. Epidermidis and saprophyticus Staphylococcus, the number of their isolates was 19, or 13.7%, and 15, or 10.8%, respectively. As for Klebsiella pneumoniae, the number of its isolates was 11, or 8%, while the number of isolates of Proteus bacteria was. vulgaris, Pseudomonas aeruginosa and Serratia.marcescens 5 isolates with a percentage of 3.7%, respectively. Mirabilis. Proteus and. Faecalis Enterococcus and Streptococcus.pneumoniae The number of its isolates was 4 and 2.9%, respectively, for Acinetobacter. baumannii, Staphylococcus.lentus and Streptococcus.pyogenes, the number of their isolates was 3 by 2.1%, while Staphylococcus.warneri bacteria were two isolates by 1.4%. The sensitivity of bacterial isolates were tested using (13) antibiotic that included quinolones, aminoglycosides, beta- lactams and tetracyclines antibiotics. Imipenem and levofloxacin are more effective, While the effectiveness of the rest of the antibiotic range from medium to ineffective The fungus Ganoderma lucidum (in terms of science/ Salah al-Din governorate north-central Iraq) was collected between August 2021-September 2021. The inhibitory activity of the secondary metabolite extract of Ganoderma lucidum was tested against twenty different types of bacteria under study, which were characterized by their multiple resistance to antibiotics. The highest inhibitory activity was against Staphylococcus. epidermidis With a diameter of inhibition of 30 mm for the aqueous extract of the old fungus by boiling for 72 hours at a concentration of 2% and at a concentration of 3%, the highest inhibitory activity against Staphylococcus. As for the inhibitory activity of the aqueous extract of the old mushroom by adding for 24 hours at a concentration of 2%, the highest inhibitory activity was against Enterococcus faecium1 bacteria with a diameter of 30 mm, and at a concentration of 3%, the highest inhibitory activity was against Serratia bacteria with a diameter of 27 mm. As for the extract of young mushrooms by steeping in boiling water for both periods 72 and 24 hours at a concentration of 3%, the highest activity was recorded against Escherichia.coil bacteria with an inhibition diameter of 36 mm. As for the secondary alcoholic extract of Ganoderma lucidum at a concentration of 100% for 24 hours, it had the highest inhibitory activity against Staphylococcus bacteria. lentus, 28 mm in diameter.