Research article

PHYLOGENETIC TREE OF MAMALIICOCUS LENTUS ISOLATED FROM STOOL SPECIMENS OF AUTISM SPECTRUM DISORDER CHILDREN IN THI-QAR PROVINCE, IRAQ

Marwa Hassan Nasser1, Qasim Hassan Widaa2, Khwam Reissan Hussein3*

Online First: November 11, 2022


Background: Staphylococcus lentus is a member of the Staphylococcus sciuri group, which that is a Gram-positive, oxidase-positive, and coagulase-negative cocci recently reclassified new genus Mammaliicoccus lentus. Autism spectrum disorder (ASD) is a neurodevelopmental disorder within which numerous neuropsychiatric disorders have been linked to the microbiota, including anxiety, schizophrenia, autism, bipolar, and depression disorder genetic ingredient that manifests itself in various distinct phenotypes. Method: Three Staphylococci lentus isolated from the stool of ASD children phenotypic identification with API Staph. The obtained nucleotide sequence of the 16S rRNA gene was processed through BioEdit software. The entire length of the 16S rRNA gene was amplified via conventional PCR using the universal 16S rRNA primer. Result: This nucleotide sequence was analyzed via the BLASTN algorithm of NCBI. All belong to Staphylococcus lentus with accessions OK614017.1, OK614015.1, and OK614016.1.The successful amplification of the total length (1500 bp) of the 16S rDNA gene was revealed. After sequencing of 16S rRNA gene, a partial fragment (800 nucleotides) was obtained.

Keywords

Autism,16S rRNA gene, Mammaliicoccus lentus