Research article

The Effect of Antibiotics and Shampoo on Different Bacterial Species Isolated From Healthy and Diseased Scalp of Humans

Mohammed H. Mohammed and Munaff J. Abd Al-Abbas

Online First: November 08, 2022

One hundred and ten bacterial isolates were obtained from 66 healthy and 44 diseased scalps from Al-Basrah peoples. 16SrRNA gene sequences identified 79 isolates of 9 species of B. cereus, S. aureus, K. pneumonia, S. pasture, S. epidermidis, E. faecalis, A. bivalvium, P. agglomerns and E. cloacae were shared in both sources, 11 species of P. pennerri, P.septica, S. sonnei, Flavobacterium sp., E. hermmmani, E. faecium, B. tropicus, B. subtilis, B. stutuzeri, A. radioresistens and A. kookiwere were only in diseased scalp comparison with 12 species of B.pumilus, S. warneri, B. altutidinis, S. marcescens, B. enrichment, C. pulveris, E. hermaachi, E. kobei, P. mirabilis, A. baumannii, P. ananatis and P. gavainiae were only in healthy scalps. In general, B. cereus and S. aureus were the most frequent in both sources. However, 9 new global strains were published in National Center for Biotechnology Information and GenBank. The phylogenetic tree showed differences in the 16SrRNA nucleotide sequence between each species isolated from the healthy and diseased scalp. Chemical shampoo appeared to be more effective (1017 colonies) to inhibit the bacteria isolates In vitro than medicated shampoo (1737 colonies). Cefotaxime, Vancomycin, Ciprofloxacin, Tetracycline and Imipenem were the best antibiotics to inhibit the bacterial species. Moreover, Erythromycin, Methicillin and Citizen were more effects toward healthy than diseased scalp isolates. The antibiotics typing for S. aureus isolates and RAPD test showed no identical strains spreading between both sources except the closely related strains No. 55- S. aureus from a diseased scalp and 83- S. aureus from a healthy scalp.


antibiotic, shampoo, scalp, 16SrRNA, RAPD