BIOLOGICAL ACTIVITY OF BACILLUS THURINGIENSIS AND GARLIC PLANT EXTRACTS ON THE WAXWORM GALLERIA MELLONELLA IN BABYLON PROVINCE

The current study was conducted of 100 soil samples from different areas in Babil Governorate, investigation analysis for bacteria Bacillus thuringiensis. According to the results shown by the study, it was found that the method, which used an inhibitor of bacterial spores, which is sodium acetate, was more selective in isolating bacteria, as the number of samples that contained B. thuringiensis bacteria when using this method was 57 samples from the total samples that were examined. The concentration of water and alcoholic extracts of garlic plant had a significant effect on the destruction of the larvae of the Great Waxworm, as the concentration treatment (3000 mg/L-1) significantly outperformed all the concentration treatments used in the research experiment and recorded the highest rate of ( 94.1, 94.9 and 79.9%) after 48, 76 hours and a week passed, respectively . The results of the current study indicated that the use of different concentrations of B. thuringiensis had a significant effect on the destruction of the larvae of the Great Waxworm, as the concentration treatment (10-2 g/ml-1) significantly outperformed all the concentration treatments used in the research experiment. The highest rate (83.3, 84.95 and 85.0%) after 48, 76 hours and a week, respectively. The results showed that there were significant differences between its treatments caused by the two factors of the study (concentration - treatment method), as the interaction treatment (concentration 10-2) with direct spraying achieved the highest rate for this trait of (90.0%) . The treatment of direct spraying outperformed the treatment of mixing with food, as it achieved the highest rate for the mentioned trait of (79.9%), while the treatment of mixing with food gave the lowest rate for this trait of (63.3%).