EFFECT OF MELATONIN AND VITAMIN A ON THE LEVEL OF SOME INFLAMMATORY MEDIATORS INDUCED BY TAMOXIFEN IN RATS

The study aimed to investigate the prophylactic effect of melatonin and vitamin A in reducing the level of some inflammatory mediators caused by the use of tamoxifen in male albino rats. 25 rats were randomly distributed in plastic cages into 5 groups. The first represented the control group, the second included treatment with tamoxifen T (80µg/kg), while the third, fourth and fifth groups included treatment with tamoxifen in addition to melatonin M (10mg/kg) and vitamin A (1500IU/kg). ) and melatonin and vitamin A synergistically, respectively. Blood samples were taken to obtain serum for laboratory tests. The results showed a significant increase (P≤ 0.01) in the concentration of monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha (TNF-α), and liver function enzymes (ALT, ALP) in tamoxifen group compared to control. The results showed a significant decrease in (MCP-1) (TNF-α) concentrations in the M, A, and M+A group compared to the T group. Melatonin showed a decrease in ALT concentration and an increase in ALP, while there were no significant differences in the AST level. While the vitamin A led to a decrease in the level of (ALP) and there was no significant difference in (ALT and AST), the synergistic treatment did not show significant differences in the level of (ALT), while an increase in (AST) and a decrease in (ALP) were observed compared to the T group. We conclude that melatonin and vitamin A has a prophylactic role against the negative effect of Tamoxifen on the physiological, immunological and biochemical variables.