SYNERGISM EFFECT OF COAGULASE ENZYME ACTIVITY WITH ANTIBIOTICS AGAINST SOME BACTERIA SPECIES

Multidrug resistance (MDR) has reached epidemic proportions in various bacterial pathogens during the past two decades.. The current study aimed to determine the activity of Coa enzyme against some types of bacteria in synergy with some antibiotics. Clinical samples were collected from patients between the ages of 18 and 45 years. Samples were collected from Al-Wasiti Teaching Hospital and Al-Kindi Teaching Hospital. The study was conducted from December 2021 to March 2022, and a hundred samples were collected, 20 from the skin, 35 from burns, 30 from wounds, and 15 from urine. The bacteria were diagnosed using the VITEC device. Minimum Inhibitory Concentration (MIC), Minimum Bactericidaӏ Concentration(MBC) and Minimum fungicidaӏ concentration (MFC) were determined for Fluconazole , Vancomycin, Gentamicin and Coagulase enzyme. Synergistic activity of coagulase enzymes with antibiotics was done . Clotting effect of Coagulase was determined. The culture results showed that out of 100 samples, 74 samples showed growth, of which 22 samples were negative for Gram stain and 52 samples were Gram positive. Bacteria growth then diagnosed by VITEC systeme the result showed (Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis and Klebsiella pneumoniae) as well as Candida albicans. The (MICs) of all antibiotics that were used during study ranged between 1 -16 µg/ml ,while MIC of coagulase extracted 0.5- 8 µg/ml . Coagulase enzyme had a synergistic effect with gentamicin against bacteria Pseudomonas aeruginosa and Enterococcus, while it did not have a synergistic effect with gentamicin against bacteria Klebsiella pneumoniae and Escherichia coli. The coagulase enzyme has the ability to inhibit and kill many species of bacteria in addition to some fungi, but it may cause human blood clots after 2 hour of incubation,in 370 .