Research article


Maria Iqbal1, Dilfaraz Khan1, Hamid Ullah2, Shafiullah Khan1*

Online First: January 30, 2023

The plant Calotropis procera was screened to evaluate its phytochemical and antioxidant capabilities in an effort to find new, potent antioxidant agents. As a result, new glycosidic Procerate ((6E,8E)-7-methyl-10-oxo-10-((3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl) oxy) deca-6,8-dienoic acid 1 was isolated from the ethyl acetate soluble fraction of Calotropis procera, along with two known compounds Octadecenoic acid 2 and Octadecadienoic acid 3 reported for the first time from C. procera. Their structures were deduced by spectral studies such as NMR, IR, UV and EIMS spectrometry. Furthermore, the antioxidant potential of the new isolated compound Procerate1 was also investigated, which showed dose-dependent antioxidant potential, where the 9 µg/mL concentration displayed highest (more than 80%) antioxidative activity in the DPPH assay. The antibacterial activity of the isolated compounds was also tested in comparison with ciprofloxacin standard against highly drug resistant bacteria Escherichia coli (E. coli) bacteria. The inhibition zones of the compounds (1-3) and ciprofloxacin are 19 ± (0.6) mm, 14± (0.1) mm, 12 ± (0.5) mm and 23 ± (0.7) mm respectively.


Calotropis procera; Isolation; Glycoside; DPPH; Antioxidant Activity; Antibacterial activity