Research article

IDENTIFYING PIRDOT LEAVES (SAURAUIA VULCANI KORTH) EFFECT ON LIVER BIOMARKER AND SPERMATOZOA QUALITY IN WHITE RATS (RATTUS NORVEGICUS) INDUCED CIGARETTE SMOKING THROUGH IN VIVO AND IN SILICO APPROACH

Erlintan Sinaga1*, Uswatun Hasanah1, Syahmi Edi1, Hendro Pranoto1, Murniaty Simorangkir2, Feimy Ruth Pratiwi Sipahutar1,2, Arman Harahap3

Online First: May 10, 2023


Cigarette smoking significantly leads to hepatocellular injury, inflammation, and infertility regarding the toxic agent and free radicals. The previous study revealed that the bioactive compound of Pirdot (Saurauia vulcani Korth.) has a high phenolic compound to be used as therapeutic agents such as immunostimulant and hepatoprotective. Our research explored more the potential of the ethanol extract of pirdot (Saurauia vulcani Korth.) (EEP) leaves on the quality of spermatozoa, hematocrit, SGPT, and SGOT of white rats (Rattus norvegicus) exposed to cigarette smoke through in vivo and silico approach. Firstly, the experiment used a completely randomized design (CRD) consisting of negative control (K-), positive control (K+), P1 treated group administered 100 mg/kg EEP; P2: 200 mg/kg EEP; and P3: 300 mg/kg EEP. Exposure to cigarette smoke was given to groups K+, P1, P2, and P3 as much as 2 stick cigarettes per day in 15 minutes. The observed data were achieved by One Way ANOVA test. Secondly, protein-protein interaction and molecular docking were utilized to investigate pathways of inhibition of bioactive compound Pirdot with CYP2A6 as protein target in nicotine metabolism. The results showed that EEP 200mg/kg BW had a significant effect on decreasing the hematocrit value and the SGPT and SGOT values. Furthermore, the white rats exposed to cigarette smoke and EEP 300 mg/kg BW had a significant effect on improving the quality of spermatozoa by increasing the morphology and motility of normal spermatozoa and elevating the concentration of spermatozoa (p0.05). Moreover, the bioactive compound Pirdot, ursolic acid, showed the promising result to bind to active site protein CYP2A6 on -14.7 kcal/mol, while methoxsalen as a comparative compound has -7.1 kcal/mol. The compound- target pathway revealed six compounds from Pirdot linked with nicotine metabolism protein which TP53 protein plays an important role in inhibiting CYP2A6 as target of smoking therapy.

Keywords

Saurauia vulcani Korth., spermatozoa quality, liver biomarker and cigarette smoking.