PREVALENCE OF BIOFILM FORMATION IN BACTERIAL PATHOGENS IN VAGINAL WOMEN INFECTIONS

Background and aims: Bacterial vaginosis (BV) is a vaginal microbiome dysbiosis that is ‎reported to be the most common gynecological condition in women of ‎reproductive age. Methods: A total 120 swab samples were collected from patients with age 18 –50 years, during the period from 7th November, 2021 to 28th February, 2022 that admitted to Al-Zahra Teaching Hospital, Al Kut Hospital, , and private clinics in Wasit province. A total 70 bacterial isolates were detected by culture characterization on the different media, biochemical investigation, analytical profile index 20 and Vitek2 automated system and investigation about biofilm production ‎using microtitre plate (MTP). Results: There were 70 bacterial-isolated samples. Results of this study has been showed that the high rate of bacterial biofilm ‎formation was in recorded as 17.1%, 14.3% and 12.9% in Escherichia coli, ‎klebsiella pneumoniae and staphylococcus epidermidis respectively, as they ‎form strong biofilm.‎biofilm formation was more prevalent in gram-negative bacteria ‎(73%), Klebsiella pneumoniae (37.41%), Escherichia coli (28.78%) Staphylococcus spp. (23.57%) was the only ‎gram-positive isolate formed strong biofilm. Conclusion: In Wasit province, there is a development in bacterial production of biofilm. This may have a harmful impact on Iraqi women and should be seen as a concerning health situation.