MOLECULAR ANALYSIS OF CYP17 GENE PROMOTER IN IRAQI INFERTILE WOMEN

Background and Aims: Female infertility is a complex multifactorial, and polygenic disease associated with genetic factors plays an essential role in its formation and follicle development, oocyte maturation, and steroidogenesis regulation in the ovary. The objective of this work was to pinpoint the polymorphism in the CYP17 gene promoter. The CYP17 gene polymorphism was discovered using DNA sequencing techniques. Results: We found that the presence of the rare allele ‘‘T’’ and heterozygous and common homozygous genotypes significantly increased the risk of infertility in women. The results detected single-nucleotide polymorphism (SNP) in the -34T>C site in the promoter of the CYP17 gene. The CYP17 gene has three genotypes according to sequence alignment, which was utilized to detect them using Geneious software V. 2020.0.4. The genotypic frequencies of TT, TC, and CC were 0.50, 0.375, and 0.125, respectively. Frequencies of T and C alleles were 0.687 and 0.313, respectively in infertility women.