EFFECT OF ORGANIC AND BIO-FERTILIZATION AND SPRAYING WITH SOME NANO-ELEMENTS ON THE FENUGREEK (TRIGONELLA FOENUM-GRAECUM L.) SEED CONTENT OF THE ACTIVE SUBSTANCE

A field experiment was conducted at the research station of the Field Crops Department - College of Agriculture - Tikrit University, and during the winter season for the years (2018-2019), Factorial experiment was conducted according to the Randomized Complete Block Design (RCBD) according to Split Plots system, and in three replications, The main plot included spraying treatments with nano elements at four levels (without addition, nano cobalt 160 mg.L-1, nano cobalt 3 mg. L-1, nan iron 160 mg.L-1 + nano cobalt 3 mg.L-1), As for the secondary plots, they included combinations between biological fertilization levels with (Sinorhizobium meliloti) bacteria at two levels (inoculation, without inoculation) and organic fertilization with decomposed sheep manure at four levels (0, 2, 4, 6) tons. dunam -1.The averages were compared using Duncan's multi-range test at (5%) level. The results showed significant differences in the seed content of the active substances (Trigonelline, Diosgenin, Gluthatione) where a result of being affected by the study factors compared to the non-fertilized treatments, as the individual treatments were achieved from foliar fertilization with the two nano elements together (iron 160 mg.L-1 + cobalt 3 mg.L-1) and 6 tons of organic fertilizer and bacterial inoculum with Rhizobium in recording the highest averages of active substances (Trigonelline): 177.61ppm, 214.06ppm, 258.54 ppm, (Diosgenin), 147.50 ppm, 172.29 ppm and 202.89 ppm, and (Glutathione) reached 1189.51 ppm, 1472.16 ppm, and 1609.00 ppm) respectively, As for the interaction between the study factors, the combination of the bacterial inoculum and organic fertilization at an average of (6) tons. dunum-1 recorded a highly significantly excelled on the seed content of the active substances, reaching (357.14 ppm), (269.96 ppm), and (2163.14 ppm) compared with the non-fertilization treatment. The fertilizer reached (6.19 ppm) (12.13 ppm) (116.18 ppm) for the active substances respectively, Through the results of the study, it is evident that foliar fertilization with chelated micro-elements with nanostructures and bio-fertilization with Rhizobium with the addition of decomposing Organic matters are necessary to create a natural balance in the nutritional status of the fenugreek plant, which is positively reflected in the increase in the seed content of the active substances.