Research article


Thuraya khaled Abdulwahed

Online First: March 14, 2023

Introduction: Recent research resulted in the development of electron transfer PCR, which employs self-quenching primers to detect different species of Plasmodium. There was some discussion about Plasmodium falciparum. The PCR assay is a reliable and user-friendly alternative to real-time PCR methods. The possibility of using polymerase chain reaction (PCR) for the molecular detection of malaria parasites in east southern Iraq. Material and Methods: DNA was isolated by QIAGEN from blood spots that had been dried out. On each of the 200 samples, PCR analysis was performed twice. Positive samples had a CT that was less than or equal to 60. Using nested PCR, 50 samples were validated for accuracy. In addition to that, a real-time PCR method based on TaqMan was used on these samples. Findings: PCR was able to identify 20 positive samples out of a total of 200. These samples were found to be positive using both nested and TaqMan-based methods. Neither PCR nor TaqMan were successful in identifying a positive sample within a subset of 50, but nested PCR was. Conclusion: Nested PCR is superior to PET-PCR in terms of sensitivity, but it is not the best choice for high-throughput sample screening. Nested PCR is a useful alternative assay for the rapid screening of a large number of samples in the laboratory because it is simple to implement and relatively inexpensive.


Malaria, molecular, east-southern, PCR, Plasmodium.