Research article


Dima Ghazi Al_Ghanimy and Lamiaa Khalifa Al_Amery

Online First: February 22, 2023

The study was conducted in the Plant Tissue Culture Laboratory of the Department of Horticulture and Landscape Engineering - College of Agricultural Engineering Sciences - University of Baghdad for the period from October 2021 to August 2022.with the aim of propagating the orchid plant Phalaenopsis amabilis (L.) tissue by determining the appropriate combination of growth regulators for all stages of propagation and studying the possibility of increasing the stimulation of some active compounds (phenolic compounds).By adding different concentrations of phenylalanine and sorbitol and comparing them with the same active compounds of the mother plant.As the single nodes were sterilized using commercial bleach (sodium hypochlorite) at a concentration of 15 ml for 15 minutes, then they were planted on MS germination medium supplemented with 60 ml L-1 coconut water, and then transferred on MS medium containing 2.5 mg L-1BA and 1 mg L- 1 NAA was added to it first with phenylalanine at concentrations of 0, 5, 10, 15 mg L-1.Secondly, the addition of the sugar alcohol sorbitol at concentrations of 0,25,50,100 g.L-1 to increase growth and stimulate active compounds (phenolic compounds). As for phenylalanine acid, the results showed that phenylalanine had an effect on vegetative growth, where the concentration of 5 mg L-1 gave the highest rate of the number of shoots (4.5 shoots.explant-1) and the number of leaves (5 leaves, explant-1). As for the length of the shoot, the concentration was given as 10 mg L-1 A at a rate of (6.5 cm). As for its effect on the active compounds (phenols)The results showed that the addition of 10 mg L-1 of the acid gave the highest concentrations of compounds (Ellagic acid, Ferulic acid, Sinapicacid, p-Coumaric acid and Benzoic acid).As for the effect of adding sorbitol concentrations, significant effects were recorded on the number of shoot, as the comparison treatment and the concentration of 25 mg L-1 were significantly superior to the rest of the concentrations by giving them the highest rate of the number of shoot (4 shoots. explant -1),As for the number of leaves, the comparison treatment gave the highest number of leaves, which reached 4 leaf .shoot-1, which did not differ significantly from the treatment 25 mg L-1 (3 leaf .shoot-1), as for the effect of sorbitol concentrations on the active compounds.The results showed that the concentration of 100 mg L-1 was significantly higher by giving it the highest concentrations of all the studied phenolic compounds (Ellagic acid, Ferulic acid, Sinapic acid, p-Coumaric acid, and Benzoic acid).As for the comparison between the active compounds of field plants and the active compounds of tissue plants, the tissue plants were significantly superior to the compounds (Ellagic acid, Ferulic acid, Sinapic acid and p-Coumaric acid).


phenylalanine, sorbitol, vegetative growth, orchids