Research article


Qasim A. Rhaif, Khansaa A. Shaheed and Balqees H. Al-Musawi*

Online First: December 21, 2022

In this study was used the PCR technique to detect the two different species of Alopecurus L. Agrostideae tribe and this technique relied on amplifying the 18s ribosomal RNA gene belonging to the chloroplast in the leaves of plants. The results of this study showed the success of the specific primer for each species in amplifying the target gene used in this study, which in turn led to the success in conducting genetic analysis of the species under study based on the ribosomal RNA gene, as it showed 99% similarities between the samples used and their genetic sequence deposited in the Genebank and compared between them. By comparing the DNA sequences of the analyzed samples with the corresponding DNA sequences, the exact locations and other details of the PCR amplified fragments were determined. A comprehensive genetic tree was generated for Alopecurus texilis and Alopecurus tiflisensis, which was based on the DNA sequences of the genetic sequences traced in the examined samples from the Agrostideae. A. texilis and A. tiflisensis, are aligned with most of the corresponding sequences in linking proximal lineages. The results also showed the presence of one mutation in the nitrogenous bases in the samples examined for each species, Furthermore, the two species were also registered in the Genebank by the researcher under the serial numbers (MZ871594.1 for A. texilis and MZ871595.1 for A. tiflisensis).


Poaceae, Alopecurus, DNA sequences, 18S ribosomal RNA gene.