Research article

THE PROTECTIVE ROLE OF BUTANOLIC CELERY SEED EXTRACT IN DIABETEC MATURE MALE RATS (STZ) HISTO-PHYSOLOGICAL STUDY

Moafaq Samir¹, Hatim A. J. Al-Shwilly²* and Qaysser A. Obaid³

Online First: December 21, 2022


To investigate the protective role of celery (Apium graveolens) seed in streptozotocin-induced (STZ) diabetes mellitus a fifty adult male Sprague-Dawely rats (240±10g) were used for this purpose, diabetes has been induced by one injection of STZ (60mg/kg) in 40 animals. plasma glucose concentration was used to confirm diabetes mellitus ( more than 200 mg/dl), after that, the animals were subjected to following treatment: Control (A): Intact rates, Diabetic group (B): diabetic rats (positive control), Diabetic extract (C): diabetic ( extract treated 30 mg/kg), (D): diabetic (extract treated 60 mg/kg), Diabetic Insulin(F): diabetic (insulin treated at a dose of 4 IU SC). After last treatment, animals were fasted 12 h., after that sacrificed by an overdose of pentobarbital (50mg/kg), samples were collected (blood and tissues), the finding showed insignificant (p≥0.05) decrease in enzymatic antioxidant status parameters catalase (CAT), glutathione (GSH) and oxidase (SOD), also, showed insignificant (p≥0.05) decrease in the liver enzymes activity AST ( aspartate aminotransferase) and ALT ( alanine aminotransferase) in treated groups compared with that of intact control and diabetic control B. Histo-pathological changes in liver showed hepatocytes necroses near central vein and pathological changes at glomeruler matrix of kidney in group positive control (B) compared with treated groups(C or D). consequently it can be said that the treated with celery seed(Apium graveolens) extract for 25 d. and 60 mg/kg dose, can mitigate stressful effect associated with diabetes mellitus.

Keywords

Butanolic celery seed, diabetec mellitus, metabolic disorder