Research article

THE CTLA4 POLYMORPHISM AND INCIDENCE OF CELIAC DISEASE IN THI-QAR PROVINCE - SOUTH OF IRAQ

Safaa Hussein Abdullah, Adnan Issa Al-Badran

Online First: December 20, 2022


Celiac disease is immune-mediated enteropathy that affects persons with genetical predisposing who develop an immune response evokes by gluten proteins, affects all ages in the same way with variable signs and symptoms. This study conducted in Thi-Qar province, the blood samples collected from one hundred healthy individuals and one hundred celiac patients whose diagnose by CD serology tests using ELISA technique. Molecular study included extraction of DNA from blood samples and amplified by PCR technique then is sequencing for CTLA4 +49A/G (rs231775) SNP to demonstrate the effect of this SNP in CD predisposing. This case control study includes (34) males and (66) females of celiac patients, ages of them range between (1 - 60) years. This study is demonstrate that Anti-tTG IgG is the best and quite accurate than other tests uses and the AG genotype of CTLA4 +49A/G (rs231775) SNP represent as risk factor for affliction with CD.

Keywords

Celiac disease, Anti-tTG, Anti-GA, Polymorphism and CTLA4.