EVALUATED THE EFFECT OF A SUB-INHIBITORY CONCENTRATION OF CLOVE OIL ON THE EXPRESSION OF APRA AND PELF GENES IN PSEUDOMONAS AERUGINOSA

The use of clove oil in the inhibition of pathogenic bacterial as well as yeast infections has been reported. The microbi-cide activity of this oil against Pseudomonas aeruginosa virulence genes was tested in this paper to estimate the effect of clove oil against the expression of Pseudomonas aeruginosa aprA and pelF genes in six wound isolates that showed highly multidrug resistance behavers. The minimum inhibitory concentrations of the oil on the bacterial isolates were identified as the six Pseudomonas isolates were treated with sub-MIC of clove oil. Then, the genes expression of aprA and pelF was detected be mean of a real-time PCR technique. The results show a significant decrease in the gene ex-pression of aprA and pelF, and their phenotypes, after treatment with clove oil.