MOLECULAR DETECTION OF SALMONELLA SPP ISOLATED FROM DIARROHEIC HUMAN, SHEEP AND GOATS IN BAGHDAD GOVERNORATE BY MULTIPLEX PCR

The current study examined the frequency of the 4 virulence genes from salmonella spp. isolated from diarrhoeic people and animals (sheep and goats) in the Baghdad governorate between October 2021 and February 2022. A total of 300 fecal samples were obtained from 100 diarrheal people, 100 sheep, and 100 goats. All faecal samples were pre-enriched in Tetrathionate broth and Rappaport Vassiliadis R10 broth before being tested for the presence of salmonella spp. After being sub cultured on SS and XLD agar, the broth culture was incubated at 37 °C for 24 to 48 hours. Standard biochemical assays and the Vitek GN test were used to identify the isolates, and multiplex PCR was used to identify the virulence genes of salmonella spp Primers were employed in the current work to amplify the (invA, fliC, fljB, and rfbJ) genes, which were chosen from four distinct genes. The findings indicated that the prevalence of Salmonella spp. was (78) isolates representing (26%) in all the samples, with sheep feces having the highest prevalence rate of 11%, followed by human feces (10%) and goat feces (5%)., 33 isolates belonged to serovar Typhimurium, 9 isolates belonged to serovar Typhi, 23 isolates belonged to serovar Enteritidis, and 13 isolates belonged to serovar arizonae Salmonella Typhi were identified by the presence of the specific amplified products to (fliC and invA) genes while S. enteritidis were identified by the presence of invA genes only while Salmonella. arizonae were identified by the presence of (invA, fljB, and rfbJ) genes. all isolates of Salmonella were positive for the invA gene amplified