EVALUATION OF HEAVY METALS CONTENTS BY ICP-MS IN WET DIGESTED FRESH WATER FISH

6.7% of total consumed high quality protein by global population comes from fish. Utilization of fish contaminated with metal causes unfavorable medical effects to human because of high penetrating probability of metal translocation in body. Current work had been done to investigate quantity of heavy metals in different fish organs by Inductively Coupled Plasma Mass Spectrophotometer (ICP-MS) after wet digestion fish from four different sites of Jehlum river, Pakistan, followed by quantification of moisture, protein, fat and ash content. Statistically analyzed results showed that heavy metal content in kidney, liver and muscles of Labeo rohita, Cattla cattla, Ctenopharyngodon idella, Cirrhinus mirgala, Hypophthalymichthys molitrix, Oreochromis niloticus, Cyprinus carpio, Wallago attu andSperata sarwari/ Sperata seenghala were in broad range, from1.09–4.7 ppb for arsenic, 0.01-0.48 ppb for cadmium, 0.05-0.32 ppb for thallium, 0.04-4.68 ppb for lead and 0.01-0.15 ppb for chromium. Proximate composition for moisture (2.5±0.8b- 80.7±4.40b %), fat (0.63±0.2d- 73.09±2.14d %), protein (1.64±0.83b- 58.35±10.49c %) and ash content (0.59±0.17a- 23.24±1.13b %) were due to broad range of fish weight and length. Since available data of heavy elements in fish is scanty, but current precise data can be used as a base for the investigation of more advanced and pharmaceutically important information.