RELATIONSHIP OF GROWTH HORMONE RECEPTOR GENE WITH SOME OF PHYSIOLOGICAL CHARACTERISTICS OF COMMON CARP CYPRINUS CARPIO L.

This study was conducted to determine the relationship of polymorphism in the growth hormone receptor gene with some of physiological characteristics (white and red blood cells, hemoglobin levels, volume of compacted blood cells, and total protein) in 45 samples of common carp (Cyprinus carpio). Below are the most important results obtained: The results of DNA sequencing, and single nucleotide polymorphism (SNP) showed there are three mutations at the following sites: C24074T, A24375G, and G24485A.The results of the blood parameters at site C24074T showed that the number of white blood cells was 136.27 ×103 cells/mm3 in the CC genotype, 131.87 × 103 cells/mm3 in the TT genotype, while it was 116.77 × 103 cells/mm3 in the CT genotype. The levels of hemoglobin were 9.23, 8.90, and 8.13 mg/100 ml in the CC, TT, and CT genotypes, respectively, and no significant differences were recorded in the average number of red blood cells, the volume of packed blood cells, and the total protein rate of the mentioned genotypes. As for the site A24375G, significant differences were recorded between the genotypes of the white blood cell count and hemoglobin levels, and no significant differences were recorded in the number of red blood cells, volume of packed blood cells, and the total protein rate, while at the site G24485A, no significant differences were recorded in all the studied characteristics. It is concluded from this study that there is a significant effect of the difference in genotypes of the growth hormone receptor gene on the physiological characteristics of common carp.