Research article

MOLECULAR IDENTIFICATION AND MORPHOLOGICAL STUDY OF GROUND BEETLE, CYMINIDIS ANDREA, MENETRIES, 1832(COLEOPTERA: CARABIDAE) FROM ERBIL GOVERNORATE KURDISTAN REGION- IRAQ

Dilzar H. Mawlud1*, Waran N. Agha1, Hero M. Muhammad1, Omer O. Hassan1, Gazang T. Omar1, Zewar Z. Omar2 and Nabeel A. Mawlood1

Online First: November 30, 2022


The present study includes a details description of the ground beetle, Cyminidis andrea Menetries, 1832 (Coleoptera: Carabidae) and its molecular identification. The specimens were collected from the underground and stones in different localities of Erbil governorate Kurdistan Region- Iraq during, March tile July 2022. According to molecular analysis, Cymindis andreae was used as a source of samples for PCR amplification of the fragments (550 bp) of the mtCOI gene for phylogenetic analysis. To match our sequence of nucleotides with sequences of other insect species that have been recorded, we exploited a section of the mtCOI gene from the collected insect which aliment the NCBI GenBank by the BLAST tool. The outcomes have gotten from the BLAST indicated that the sequences obtained newly identified species based on the mitochondrial COI gene and recorded as a new species in GenBank. The identified Cymindis andreae in the Kurdistan region clustered in a polyphyletic clade available from other countries. The COI sequence of Cymindis andreae was submitted to GenBank with accession OP361098. The base pairing of the mitochondrial COI gene sequence was highly adenine & thymine bps.mt COI gene can be practiced as a marker for the detection of related species, as detected from the likenesses between the phylogenetic association constructed by COI and morphological facts. The morphological study showed that the diagnosis characteristics of the species are, mandibles triangular, apically with a single tooth. Third segment of labial palps securiform, 1.4 times as long as 2nd segment. Antenna filiform, 1st antennomere cylindrical, 2 times as long as 2nd. Elytra dark yellow, surface with seven striae. Protibial tubular shaped, inner margin with cleaning organ. Right paramere nearly rounded, apical, and inner margin without setae. The habitus and diagnostic parts of the species were photographed. We achieved that the

Keywords

Molecular identification; morphological study; mt COI gene; Cyminidis andrea; sequencing.