CORRELATION BETWEEN SERUM LEVEL OF LOW-DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN AND ITS SINGLE GENE POLYMORPHISMS IN TYPE 2 DIABETES MELLITUS

Diabetes mellitus is a metabolic disease described as the presence of hyperglycemia because of failure in insulin secretion, defective action of insulin, or both. In this study, serum levels of LRP1 and genetic polymorphism (rs1800127) of the LRP1 gene were investigated in Type 2 diabetes (T2D) patients and healthy controls to determine their disease-associated risk. Allele-specific primer technique was used to determine the polymorphisms. Accordingly, a case-control analysis was carried out on 50 patients and 50 control from October 2020 – December 2020. The patients and control were characterized for age, sex, Body Mass Index (BMI), HbA1c, and lipid profile, including cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein, and very low-density lipoprotein. The results are given in the following: The mean age of patients increased significantly compared to the control (50.22 ±9.34 vs. 44.28 ±9.28 years; p < 0.0019). At the same time, the mean BMI of T2D patients was insignificant compared to controls (30.39 ±2.64 vs. 30.96 ±4.57 kg/m2; p ≤ 0.452). Furthermore, there was no significant difference in LRP1 level in patients with T2DM compared with controls (49.19±18.45 vs. 33.43 ±12.66 pf/mL; p < 1.00). Genetic analysis of rs1800127 SNP of the LRP1 gene revealed significant differences (p =0.0091) between the observed and expected genotypes. In CT Polymorphism, the odds ratio for the CT genotype was 3.16 with p=0.066 indicating that individuals who carry CT are more likely to encounter T2DM, which means that the SNP (rs1800127) has a role in the incidence of the disease. Moreover, the data detected significant variations (p=0.0347) between the observed and expected genotypes of rs7968719. In addition, In CG Polymorphism, the odds ratio for the CG genotype was 0.07 with p-value=1, indicating that there was non-significant CG genotyping of T2DM patients compared with controls. The odd ratio of genotype frequencies of allele G was 1.38, which suggests that the G allele may have a critical role in the incidence of T2DM.