PHYTOCHEMICAL SCREENING AND IN VIVO ANTI-ANEMIC ACTIVITY OF SOME MEDICINAL PLANTS

Background: More than two billion people around the world suffer from anemia. Some people resort to eating medicinal herbs that have an effective anti-anemic effect. It is estimated that 239 million children and 468 million non-pregnant women suffer from anemia globally. Anemia is a common nutritional deficiency among all age groups in Pakistan, which accounts for 83% of all anemic patients. Anemia is one of the most common medical problems, especially in developing countries. It is preferable to use a diet rich in iron supplements, but taking iron orally causes many problems due to the difficulty of absorption due to an imbalance in the digestive system. Liver and kidneys. Therefore, some medicinal plants have been known as anti-anemic. Aim of the work: This project is an initiative to investigate the anti-anemic potential of traditionally used medicinal plants i.e. Terminalia chebula, Terminalia bellirica, Syzygium cumini, Phyllanthus emblica, Piper nigrum Linn, Amomum subulatum, Cuminum cyminum. Material and methods: Furthermore, a coded herbal formulation were developed in the current study to evaluate the chemical and biological properties such as qualitative and quantitative phytochemical analysis, in vitro antioxidant activity, acute and subacute toxicity testing, in vivo anti-anemic activity. Results: These plants are rich in phenols, flavonoids, and iron. In vivo activity can be performed, firstly acute toxicity test was performed in which a mortality rate of mice was 20% to 40% at 4000 mg/kg detected. Conclusion: These plants were shows strong anti-anemic activity. This research project moves toward an alternative hypothesis. In further research, clinical trials were performed to assess their result in patients.