Research article

EFFICACY OF SILVER NANOPARTICLES ACTION AND COMPARISON WITH SOME ANTIBIOTICS ON E.COLI BACTERIA ISOLATED FROM CLINICAL AND MEDICAL WASTE SAMPLES IN THE-QAR PROVINCE

1Bushra Ali Ghyadh, 2Manal Badi Saleh

Online First: October 29, 2022


Silver nanoparticles (Ag NPs) are well famous for their antimicrobial effects against a extensive diversity of drug-resistant microorganisms , Ag NPs were established against pathogenic Escherichia coli UTI and E. coli isolated from medical waste in some hospitals in Thi-Qar province south of Iraq . Antimicrobial results were shown by concentration (0.1,1and 3) mM of Ag NPs against E. coli, the maximum concentration action effect on E. coli was 3mM .The antibiotics susceptibility experiment was done [Ampcillin+Clavulanic acid (AMC), Ampcillin (AM) , Impenime (IMP), Cefotaxime (CTX), Ciprofloxin (CIP), Tetracycline (TE) and Trimethoprim (TMP)] . The results revealed that a significant increase of antibiotics resistant at p. value < 0.05 in E. coli bacteria isolated in medical waste than these isolated from urine sample most tested antibiotics ,results detailed a significant difference of antibiotics activity at p. value < 0.05, the IMP, CIP and CTX were recorded the high antibacterial activity in both E. coli isolated. We note that the inhibition of silver nanoparticles (3mM) on E. coli bacteria has more effect compared with antibiotics (TMP ,TE,AM,AMC),while less than ( IMP,CTX ,CIP)., The results of the present study indicated that the wound length reduced in experimental mice during treatment with silver nanoparticles at a concentration of 0.06µg was more effective than 0.03 µg

Keywords

silver nanoparticles, antibacterial activity