Research article

EFFECT OF SOME PESTICIDES ON 1-AMINOCYCLOPROPANE-1- CARBOXYLATE (ACC) DEAMINASE BIOSYNTHESIS GENE OF DROUGHT RESISTANCE PSEUDOMONAS PUTIDA AND TRANSCONJUGANT PSEUDOMONAS FLUORESCENS

Karzan Kazm Mahmud1*, Aras Muhammad Khudhur2, Goran Noori Saleh3, Muzhda Haydar Saber4, Sakar B. Smail6 and Abdulkarim Yasin Karim5

Online First: February 22, 2023


This research was conducted to evaluate the effects of four main usable pesticides in Kurdistan Region – Iraq on plant growth promoting and drought resistance bacteria Pseudomonas putida and transconjugant Pseudomonas fluorescens contained 1-aminocyclopropane-1- carboxylate (ACC) deaminase gene. The most usable pesticides in the region were Glyphosate, Topsin, Vantex and Velum prime used at the recommended concentrations in bacterial culture media and soil. According to the results, herbicide Glyphosate did not have notable effects on the both bacterial numbers and viability of 1-aminocyclopropane-1- carboxylate (ACC) deaminase gene in the genomes of Pseudomonas putida and transconjugant Pseudomonas fluorescens. However, other pesticides were exhibited inhibitory effects on the growth of tested bacteria in culture media and soil. Furthermore, negative effects were revealed on the viability of the gene after treating with Vantex and Velum prime pesticides in culture media. While, there was the gene viability in Pseudomonas putida genomes treated with Topsin in culture media and soil. The results illustrated that Glyphosate does not have significant effects on Pseudomonas putida and transconjugant Pseudomonas fluorescens bacterial communities and genomes, whereas the other three pesticides have negative impacts on the both bacterial communities and both bacterial genomes especially Vantex and Velum prime. Therefore, it has been suggested that using Topsin, Vantex and Velum prime pesticides should be limited or avoided for controlling plant fungi, insects and nematodes respectively.

Keywords

Pseudomonas putida strain UW4, Transconjugant Pseudomonas fluorescens, Pesticides, Bacterial numbers, ACC deaminase gene.